Table 2.
Sensitivity of Saci_1096 mutant to DNA-damaging agentsa.
| Damaging chemical | Type of damage | DG185 (con) | DG251 (con) | LR12 |
|---|---|---|---|---|
| cisplatin (cis platinum(II) diammine dichloride) | Intra- and interstrand crosslinks | 37.5 ± 0.0 | 37.5 ± 0.0 | 37.5 ± 0.0 |
| MNNG (N-methyl N′-nitro N-nitrosoguanidine) | Base methylation | 43.8 ± 28.6 | 43.8 ± 28.6 | 43.8 ± 28.6 |
| Mechlorethamine (2-chloro-N-(2-chloroethyl)-N-methyl-ethanamine) | Intra- and interstrand crosslinks | 250.0 ± 86.6 | 250.0 ± 86.6 | 250.0 ± 86.6 |
| Metronidazole (2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethanol) | Bulky adduct | 2.5 × 103 ± 0 | (no data) | 2.5 × 103 ± 0 |
| 4-nitroqunoline N-oxide | Bulky adduct | 0.4 ± 0.17 | 0.5 ± 0.17 | 0.5 ± 0.17 |
| Butadiene diepoxide | Intrastrand crosslinks | 100 ± 43.3 | 100 ± 43.3 | 100 ± 43.3 |
| Hydrogen peroxide | Oxidation and strand breaks | 93.8 ± 44.2 | 93.8 ± 44.2 | 93.8 ± 44.2 |
a The average MIC values ± standard deviation (μg/mL) were calculated from 3 replicates, except for hydrogen peroxide (average of two replicates).