Figure 5.

Spatiotemporal changes of PI fluorescence and [Ca²⁺]_i in surviving and non-surviving cells affected by US (1.25 MHz, 10 cycles, 0.24 MPa). (A, B) PI and [Ca²⁺]_i in a reversibly sonoporated cell, (C, D) PI and [Ca²⁺]_i in an irreversibly damaged cell, (E) a cell that was dead before US. The pre-US PI images in (A), (C), and (E) are superimposed on the brightfield image taken prior to the start of fluorescence imaging. The color bar in each column indicates PI fluorescence intensity in arbitrary units and the [Ca²⁺]_i in nM. The scale bar indicates 25 μm.