Table III. dT8 and dT16 tracking parameters in wild-type and mutant PcrA proteins.
(a) dT8 tracking parameters in wild-type and mutant PcrA proteins | ||||
---|---|---|---|---|
Protein | Rapid phase rate (s–1) | Steady state rate (s–1) | Rapid phase duration (ms) | Rapid phase magnitude (Pi/PcrA) |
Wild type | 34 | 6.1 | 94 | 3.1 |
K137A | 23 | 4.8 | 125 | 2.9 |
K138A | 13 | 5.2 | 125 | 1.7 |
K419A | 25 | 6.4 | 114 | 2.8 |
T426A | 29 | 5.8 | 135 | 3.7 |
K456A | 29 | 5.4 | 101 | 2.9 |
K419A/K456A |
27 |
5.2 |
106 |
2.9 |
(b) dT16 tracking parameters in wild-type and mutant PcrA proteins | ||||
Protein |
Rapid phase rate (s–1) |
Steady state rate (s–1) |
Rapid phase duration (ms) |
Rapid phase magnitude (Pi/PcrA) |
Wild type | 43 | 6.3 | 160 | 6.9 |
K137A | 27 | 5.5 | 219 | 5.9 |
K138A | 24 | 5.7 | 117 | 2.9 |
K419A | 34 | 6.8 | 184 | 6.2 |
T426A | 34 | 5.9 | 200 | 6.7 |
K456A | 40 | 5.5 | 151 | 6.0 |
K419A/K456A | 35 | 5.7 | 165 | 5.7 |