Fig. 3. Effect of PtsG-containing membranes on Mlc binding to its target DNA. The DNA fragment, Glc1–4 (Figure 1) labelled at Glc4, was mixed with Mlc (lanes 2–8) (extract from an overproducing strain, ∼30 µg/ml), and PtsG-containing membranes (lanes 3–6) or control membranes (lanes 7–8) were added (lanes 3 and 7, 100 µg/ml; lanes 4 and 8, 50 µg/ml; lane 5, 25 µg/ml; lane 6, 12.5 µg/ml). Complexes were analysed on small native 5% polyacrylamide gels. The positions of migration of free DNA and DNA complexed with Mlc (Bound) are indicated.