Table III. Effect of plasmids carrying IICGlc and IIBGlc domains on expression of ptsG-lacZ during growth on glucose.
Plasmid | ptsG-lacZ | |
---|---|---|
Gly | Glc | |
pTZ(PtsG) | 9.5 | 39 |
pACH (IICGlcHis6) and pTZ(PtsGΔ5–320) | 12.5 | 33 |
pACH (IICGlcHis6) and pJBH (IIBGlcHis6) | 9.5 | 11 |
pACH (IICGlcHis6) | 15 | – |
JM-G96 (ΔptsG, ptsM8) was transformed with the plasmids indicated and β-galactosidase activities measured during growth in minimal medium with glycerol (0.4%) or glucose (0.2%), 0.1 mM IPTG, 0.5 mg/ml ampicillin for cultures with pTZ(PtsG), pTZ(PtsGΔ5–320) or pJBH and 40 µg/ml kanamycin for cultures with pACH. Results are the mean of three independent cultures.