. 2000 Oct 16;19(20):5353–5361. doi: 10.1093/emboj/19.20.5353

Table III. Effect of plasmids carrying IIC^Glc and IIB^Glc domains on expression of ptsG-lacZ during growth on glucose.

Plasmid	ptsG-lacZ
	Gly	Glc
pTZ(PtsG)	9.5	39
pACH (IIC^GlcHis₆) and pTZ(PtsGΔ5–320)	12.5	33
pACH (IIC^GlcHis₆) and pJBH (IIB^GlcHis₆)	9.5	11
pACH (IIC^GlcHis₆)	15	–

JM-G96 (ΔptsG, ptsM8) was transformed with the plasmids indicated and β-galactosidase activities measured during growth in minimal medium with glycerol (0.4%) or glucose (0.2%), 0.1 mM IPTG, 0.5 mg/ml ampicillin for cultures with pTZ(PtsG), pTZ(PtsGΔ5–320) or pJBH and 40 µg/ml kanamycin for cultures with pACH. Results are the mean of three independent cultures.

Table III. Effect of plasmids carrying IICGlc and IIBGlc domains on expression of ptsG-lacZ during growth on glucose.

Table III. Effect of plasmids carrying IIC^Glc and IIB^Glc domains on expression of ptsG-lacZ during growth on glucose.