Fig. 7.

ChIP analysis showing in vivo binding of AP-2β to the AP2 binding sites of the rat PNMT promoter. (A) Schematic drawing of the three AP2 binding sites (i, ii, iii) in the rat PNMT promoter as described (Ebert et al., 1998). Primer sets to detect the AP2 binding sites and the size of expected PCR products are shown. The bent arrow represents the PNMT transcription start site. The first PNMT exon is shown (Exon I). (B) The protein-DNA complexes were immunoprecipitated using antibodies against AP-2β (lane 4, 9). Normal rabbit IgG was used as a negative control (lane 3, 8). PCR was performed without template and used for PCR control (lane 2, 7). Input DNA (lane 1, 6) and 300 bp DNA size marker (lane 5, 10) are shown. PCR products were analyzed by electrophoresis on 7% polyacrylamide gel.