FIG. 5.

Comparison of the amniotic fluid-derived stem (AFS) cells ability to formation of capillary-like structures in Matrigel between static and shear stress by in vitro angiogenesis assay. (A) Representative images captured after plating on Matrigel for 2, 8, and 24 h. AFS cells cultured in endothelial growth media-2 for 2 weeks and subsequently incubated under static conditions or exposed to shear stress (12 dyne for 2, 8, or 24 h) were seeded onto Matrigel. Capillary-like structures appeared at 8 h in static AFS cell cultures, while this process began as early as 2 h in shear-stressed cells. (B) Quantification of tube formation. National Institutes of Health image was used to determine the total length of the tube-like structures in images of cells exposed to shear stress for 2, 8, and 24 h (*P < 0.01 vs. static control, n = 3).