Fig. 7. Nuclear accumulation of UbcM2 is dependent on functional importin-11. BHK cells ectopically expressing Ubc-GG alone (–) or together with zz-tagged importin-11 (ZZ-Imp-11), the mutant ZZ-Imp-11(78–975) or importin-β were processed for immunofluorescence as described for Figure 3. GFP was imaged under conditions such that no pixels were saturated, and quantitated to obtain total fluorescence (N+C) and nuclear fluorescence (N) for each cell. Graphs show fractional nuclear GFP fluorescence for a range of expression levels of the Ubc-GG, in the presence or absence of the co-expressed importins. The data in the graphs were compiled from 10–20 cells in each condition for two separate experiments. Bar, 10 µm. (See Supplementary data for method of image analysis and importin-β graph.)