Figure 2.

Downregulation of RBM3 affects the expression of MCM3, Chk1, and Chk2. The expression of MCM3, Chk1, Chk2, and RBM3 were examined by (A) Western blot analysis and (B) reverse transcription-PCR in A2780 cells 48 hours after transfection of cells with three different siRNAs targeting RBM3 (nos. 58, 59, and 60). Data shown are mean ± SEM of four, for siRBM3 nos. 58 and 59, and three for siRBM3 no. 60, independent experiments performed in triplicate. (C) Immunohistochemical staining of Chk1, Chk2, and MCM3 in EOC tumors denoted as negative, intermediate, and strong.