Figure 4.

The impact of RBM3 on phosphorylation of Chk1 and Chk2 and the association of pSer345-Chk1 and pT68-Chk2 with OS in EOC. The impact of RBM3 on checkpoint response was examined in siRBM3-transfected cells by Western blot analysis using antibodies against (A) pSer345-Chk1 and pT68-Chk2. (B) Immunohistochemical staining of pSer345-Chk1 and pT68-Chk2 in EOC tumors. (C) Kaplan Meier analysis of OS according to immunohistochemical staining of pS345-Chk1 and pT68-Chk2 in cohort 2 in strata defined as high versus low expression.