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. 2011 Aug 1;4(4):234–240. doi: 10.1593/tlo.11115

Table 1.

CXCR4 Expression on MDAMB231 and MDAMB231CXCR4+ Cells Incubated at Different Conditions (n = 3–6).

Cell Type Signal Intensity Difference MDAMB231 and MDAMB231CXCR4+

MDAMB231 MFIR ± SD MDAMB231CXCR4+ MFIR ± SD
12G5-PE
Live cells; on ice 1.116 ± 0.054 4.920 ± 1.463 4.4*
Live cells; RT 1.160 ± 0.108 8.747 ± 1.245 7.5
Fixed cells; on ice 7.380 ± 3.573 11.046 ± 2.143 1.5
2B11-PE
Live cells; on ice 1.179 ± 0.072 1.487 ± 0.051 1.3
Live cells; RT 1.206 ± 0.047 1.435 ± 0.042 1.2
Fixed cells; on ice 18.814 ± 6.636 13.378 ± 3.267 0.7
Ac-TZ14011-FITC
Live cells; on ice 2.337 ± 0.341 5.505 ± 0.900 2.4
Live cells; RT 2.970 ± 0.289 4.941 ± 0.487 1.7*
Fixed cells; on ice 25.788 ± 1.575 62.357 ± 7.920 2.4

For significance: MDAMB231 MFIRs were compared to MDAMB231CXCR4+ MFIRs.

*

P ≤ .05.

P ≤ .005.