Table 1.
CXCR4 Expression on MDAMB231 and MDAMB231CXCR4+ Cells Incubated at Different Conditions (n = 3–6).
Cell Type | Signal Intensity Difference MDAMB231 and MDAMB231CXCR4+ | ||
MDAMB231 MFIR ± SD | MDAMB231CXCR4+ MFIR ± SD | ||
12G5-PE | |||
Live cells; on ice | 1.116 ± 0.054 | 4.920 ± 1.463 | 4.4* |
Live cells; RT | 1.160 ± 0.108 | 8.747 ± 1.245 | 7.5† |
Fixed cells; on ice | 7.380 ± 3.573 | 11.046 ± 2.143 | 1.5 |
2B11-PE | |||
Live cells; on ice | 1.179 ± 0.072 | 1.487 ± 0.051 | 1.3† |
Live cells; RT | 1.206 ± 0.047 | 1.435 ± 0.042 | 1.2† |
Fixed cells; on ice | 18.814 ± 6.636 | 13.378 ± 3.267 | 0.7 |
Ac-TZ14011-FITC | |||
Live cells; on ice | 2.337 ± 0.341 | 5.505 ± 0.900 | 2.4† |
Live cells; RT | 2.970 ± 0.289 | 4.941 ± 0.487 | 1.7* |
Fixed cells; on ice | 25.788 ± 1.575 | 62.357 ± 7.920 | 2.4† |
For significance: MDAMB231 MFIRs were compared to MDAMB231CXCR4+ MFIRs.
P ≤ .05.
P ≤ .005.