Fig. 6.

DES, but not BPA, exposure in utero increases EZH2 mRNA expression in adult murine mammary tissue. Quantitative RT-PCR was performed in mammary tissue from 6-week-old mice after in utero exposure to DES or BPA during days 9–26 of gestation. DES treatment resulted in a 2-fold increase in EZH2 mRNA expression when compared with control mice. BPA treatment did not alter expression of EZH2 mRNA. Results are representative of three independent experiments using five animals in each treatment group in each experiment. *p < 0.05.