Fig. 3. B-RafT598A, B-RafS601A or B-RafAA does not alter the association with 14-3-3, HSP90 or MEK. COS cells were transiently transfected with pcDNA3 (vector, lane 1), HA-B-Raf (lane 2), HA-B-RafT598A (lane 3), HA-B-RafS601A (lane 4) or HA-B-RafAA (lane 5). Cells were lysed and the HA-tagged B-Raf was immuno precipitated (IP) with anti-HA and western blotted with anti-HA (B-Raf), anti-14-3-3, anti-HSP90 or anti-MEK antibodies.