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. Author manuscript; available in PMC: 2011 Jul 20.
Published in final edited form as: J Cell Biochem. 2009 Oct 1;108(2):433–446. doi: 10.1002/jcb.22270

Figure 3. Role of nAChR subunits in human microvascular EC migration.

Figure 3

(A) Representative microphotographs of the HMVEC migration assay. Nicotine (10−8M) stimulates migration of HMVEC into denuded area (Right panel). Bar = 50 µm (B) Effect of silencing individual nAChR on HMVEC migration induced by nicotine (10−8M), or VEGF (10ng/ml). Endothelial cells exposed to scrambled siRNA were used as a control for the effects of transfection. Values are expressed as a percentage of migrating cells per high-powered field (n=12) in vehicle-treated wells with error bars showing SEM. * *p<0.05 versus vehicle treated cells. # p<0.05, vs. non-transfected cells exposed to same stimulus.