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Immunoblotting for p21 and cyclin D1. Cells were exposed to siRNA against α9 or α7 for 72 hour. Then cells were serum starved overnight and subsequently stimulated with nicotine (10−10M) for 24 hours. The lysates were subjected to Western analysis with anti-p21, anti-cyclin D1 antibodies. Equal protein loading was determined by anti-β-actin antibody.
