A) Splenic CD4+ T cells and CD19+ B cells were profiled on Affymetrix, Agilent, Nimbelgen and Illumina whole-genome microarrays. Resulting gene-expression data from each platform were analyzed to yield the percentage of expressed probes, percentage of false-positives (defined as a probe being expressed on one platform, but not the other three), percentage of false-negatives (defined as the absence of a probe's expression in one platform but present in the other three) and overall concordance (defined as the overall percentage of probes whose expression or absence is in agreement with the majority of platforms). B) The overall expression of the genome in T and B cells was calculated based on the number of genes registering as significantly expressed for each platform with concordance being defined as a given gene's expression or absence in 2, 3 or 4 out of 4 platforms (rows).
