Fig. 7. Detection of RtxA in concentrated culture supernatant fluids by western blotting. A 2.6 µg aliquot of concentrated culture supernatant fluids from KFV43 (RTX+) and CW128 (RTX–) was loaded in each lane. RtxA was detected using pre-immune rabbit serum (left) or serum raised against RtxA138 (right). Arrows on the right denote the location of RtxA-specific bands. Those marked with an asterisk denote the three major breakdown products.