Table 4.
TOP2A, MCM2, and BUB1B protein expression scores in IDCs, molecularly low-risk and molecularly high risk, histologically normal breast tissues.
| Case no. | Breast tissue specimen category (based on gene expression profiling) | Histologic tumor type on initial biopsy/lumpectomy | Final pathologic diagnosis on mastectomy | Histopathologic findings on archival tissue sections selected for IHC validation | Topoisomerase II-alpha (TOP2A) index (%) | MCM2-index (%) | BUB1B protein expression score (H-score) |
|---|---|---|---|---|---|---|---|
| 1 | Carcinoma | IDC | IDC, DCIS | Invasive Cancer | 25 | 30 | 170 |
| 2 | Carcinoma | IDC | Invasive papillary CA with a focus of IDC | Invasive Cancer | 30 | 30 | 135 |
| 3 | Carcinoma | IDC | IDC | Invasive cancer | 15 | 80 | 145 |
| 4 | Carcinoma | IDC | IDC | Invasive Cancer | 35 | 60 | 80 |
| 5 | Carcinoma | IDC | IDC | Invasive Cancer | 25 | 50 | 165 |
| 6 | Carcinoma | IDC, ILC | IDC w/ lobular features | Invasive cancer | 30 | 30 | 200 |
|
| |||||||
| HNB tissues with low-grade molecular abnormality (HNB-LGMA) | |||||||
|
| |||||||
| 7 | HNB-LGMA 1 | IDC | IDC | Benign breast tissue | 2.5 | 2 | 21 |
| 8 | HNB-LGMA 2 | IDC | IDC | Benign breast tissue | 2.5 | 6 | 20 |
| 9 | HNB-LGMA 3 | IDC | IDC | Benign breast tissue | 2.5 | 12 | 22 |
| 10 | HNB-LGMA 4 | Mucinous carcinoma | No residual mucinous carcinoma | Benign breast tissue | 1 | 2 | 15 |
| 11 | HNB-LGMA 5 | DCIS | Residual DCIS, multifocal | Benign breast tissue | 1 | 1 | 10 |
| 12 | HNB-LGMA 6 | IDC | No residual IDC | Benign breast tissue | 1 | 1 | 18 |
| 13 | HNB-LGMA 7 | DCIS | Residual DCIS | Benign breast tissue | 2 | 2 | 15 |
| 14 | HNB-LGMA 8 | DCIS | No residual DCIS | Benign breast tissue | 3 | 8 | 11 |
| 15 | HNB-LGMA 9 | DCIS | Residual DCIS | Benign breast tissue | 1 | 1 | 17 |
|
| |||||||
| HNB tissues with high-grade molecular abnormality (HNB-HGMA) | |||||||
|
| |||||||
| 16 | HNB-HGMA 1 | Adenoid cystic carcinoma | No residual adenoid cystic carcinoma | Benign breast tissue | 8 | 12 | 33 |
| 17 | HNB-HGMA 2 | DCIS | DCIS, multifocal | Benign breast tissue | 12.5 | 20 | 64 |
| 18 | HNB-HGMA 3 | Intracystic carcinoma | No residual intracystic carcinoma | Benign breast tissue | 12.5 | 8 | 35 |
| 19 | HNB-HGMA 4 | IDC | IDC focal papillary features | Benign breast tissue | 9 | 30 | 113 |
| 20 | HNB-HGMA 5 | IDC | IDC | Benign breast tissue | 2 | 12 | 46 |
| 21 | HNB-HGMA 6 | IDC | No residual IDC | Benign breast tissue | 15 | 30 | 46 |
| 22 | HNB-HGMA 7 | No prior biosy performed | Benign breast tissue-patient BRCA1+, strong family history of BC | Benign breast tissue | 10 | 25 | 106 |
| 23 | HNB-HGMA 8 | DCIS | No residual DCIS | Benign breast tissue | 4 | 12 | 43 |
| 24 | HNB-HGMA 9 | IDC, ILC | No residual invasive carcinoma | Benign breast tissue | 30 | 35 | 94 |