Table 3.
Forebrain expression of GAD65 mRNA(corrected gray levels)
| Control
|
CVS
|
|||||
|---|---|---|---|---|---|---|
| Water | Saccharin | Sucrose | Water | Saccharin | Sucrose | |
| mPOA | 38.6 ± 3.3 | 45.0 ± 2.9 | 40.2 ± 1.3 | 47.5 ± 3.7 | 40.9 ± 3.1 | 42.3 ± 3.4 |
| ZI | 36.3 ± 2.9 | 39.5 ± 1.0 | 33.2 ± 2.1 | 30.9 ± 2.1 | 35.7 ± 1.9 | 35.5 ± 2.6 |
| BSTpd ab | 43.1 ± 1.5 | 42.0 ± 2.0 | 36.9 ± 1.9# | 48.2 ± 2.2 | 43.9 ± 1.6 | 43.5 ± 2.9* |
| BSTpv a | 34.0 ± 1.8 | 34.6 ± 1.9 | 31.0 ± 1.7 | 36.8 ± 1.9 | 34.7 ± 1.6 | 38.4 ± 3.4* |
| BSTad | 26.0 ± 1.2 | 26.8 ± 1.6 | 26.8 ± 1.6 | 26.0 ± 1.6 | 24.3 ± 1.1 | 26.1 ± 1.2 |
| BSTav | 28.1 ± 1.5 | 28.3 ± 1.4 | 27.6 ± 2.3 | 29.3 ± 1.2 | 27.3 ± 1.3 | 31.0 ± 1.1 |
| NAc core | 21.6 ± 1.2 | 24.1 ± 1.1 | 22.1 ± 1.4 | 22.3 ± 1.6 | 22.7 ± 1.5 | 23.1 ± 1.8 |
| NAc shell | 27.2 ± 1.7 | 31.3 ± 1.4 | 29.8 ± 2.6 | 30.5 ± 2.5 | 30.0 ± 1.9 | 31.0 ± 2.5 |
| DG | 25.2 ± 1.7 | 20.5 ± 1.4 | 21.4 ± 2.6 | 23.5 ± 1.2 | 23.1 ± 1.9 | 23.2 ± 1.1 |
Additional regions expressing forebrain pro-dynorphin. An a indicates regions with a main effect of stress, a b indicates regions with a main effect of drink p<0.05. An * indicates a significant difference from non-CVS control and a # indicates a significant difference from water control by post hoc analysis. Data are shown as mean ± SEM with n=5-9 per group. List of abbreviations: chronic variable stress (CVS), medial preoptic area (mPOA), zona inserta (ZI), bed nucleus of the stria terminalis posteriordosal subregion (BSTpd), bed nucleus of the stria terminalis posterioventral subregion (BSTpv), bed nucleus of the stria terminalis anterodorsal subregion (BSTad), bed nucleus of the stria terminalis anteroventral subregion (BSTav), nucleus accumbens core (NAc core), nucleus accumbens shell (NAc shell), dentate gyrus of hippocampus (DG).