Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Mol Biol. 2011 May 5;410(1):50–59. doi: 10.1016/j.jmb.2011.04.070

Figure 5.

Figure 5

Determination of the completion of DNA packaging. Particles containing wild-type (lanes 1 and 5), the triple-A mutation (lanes 2 and 6) or loop deletion of (lanes 3 and 7) were added to the packaging system. After 15 min. incubation, the reaction was split and γ-S-ATP added to one part to stall the motor. DNase was then added to digest the unpackaged DNA, and the packaged DNA was extracted and cut with ApaLI. Lanes 1–3 were derived from the standard packaging samples (no γ-S-ATP addition). Lanes 5–7 were derived from the portion of the reaction that was stalled with γ-S-ATP. Lane 4 and 8 were control digests of φ29 DNA alone treated with ApaLI ; markers=100 bp DNA ladder.