Table 2. Characterisation of 15 microsatellite markers in 24 unrelated foxes from Bristol's high-density population.
Marker | Multiplex set | N | Florescent label | Alleles observed | Allele size range (bp) | HWE p-value | Non-exclusion probability parent pair | Estimated frequency of null alleles | HObs | HExp |
FH2541 | 1 | 23 | HEX | 8 | 170–206 | 0.5691 | 0.2122 | +0.0530 | 0.783 | 0.814 |
REN69B24 | 1 | 20 | 6-FAM | 7 | 228–280 | 0.7865 | 0.2293 | −0.0391 | 0.750 | 0.771 |
REN161A12 | 1 | 22 | HEX | 5 | 295–303 | 0.8629 | 0.4392 | −0.0680 | 0.682 | 0.608 |
V374 | 1 | 23 | HEX | 4 | 110–116 | 0.6962 | 0.3335 | −0.0647 | 0.739 | 0.738 |
V468 | 1 | 21 | 6-FAM | 5 | 83–93 | 0.6550 | 0.2844 | −0.0609 | 0.762 | 0.736 |
V602 | 1 | 23 | 6-FAM | 5 | 137–162 | 0.1400 | 0.3753 | +0.0281 | 0.609 | 0.663 |
DGN3 | 2 | 22 | 6-FAM | 9 | 192–250 | 0.7768 | 0.1517 | −0.0177 | 0.864 | 0.854 |
DGN14 | 2 | 18 | HEX | 7 | 224–250 | 0.7546 | 0.2102 | +0.0105 | 0.722 | 0.811 |
REN162B09 | 2 | 24 | HEX | 2 | 190–194 | 1.0000 | 0.6793 | −0.0237 | 0.500 | 0.507 |
V142 | 2 | 23 | HEX | 6 | 131–143 | 0.0906 | 0.2945 | +0.0634 | 0.652 | 0.727 |
FH2174 | 3 | 20 | HEX | 9 | 232–276 | 0.6574 | 0.1690 | +0.0102 | 0.850 | 0.831 |
FH2658 | 3 | 13 | 6-FAM | 14 | 352–449 | 0.2187 | 0.0677 | +0.0682 | 0.846 | 0.938 |
FH2281 | 4 | 23 | 6-FAM | 9 | 429–465 | 0.8526 | 0.1571 | −0.0777 | 0.957 | 0.849 |
FH2309 | 4 | 23 | 6-FAM | 5 | 350–370 | 0.2808 | 0.2980 | +0.0754 | 0.652 | 0.766 |
FH2316 | 4 | 21 | HEX | 11 | 282–368 | 0.9346 | 0.1232 | −0.0582 | 1.000 | 0.878 |