Table 3.
Comparison of expression of the SRF-modulated genes containing classic CArG elements between mild overexpression SRF transgenic mouse and other mouse models. The cardiac gene microarray data of 27 experiments were downloaded from the Gene Expression Omnibus (GEO) database. The expression of SRF-modulated genes was compared among mild-SRF transgenic (Tg) mice and other mouse models in the GEO database that used the same Affymetrix gene chip as in our experiment. The majority of these mouse models used in comparison also had hemodynamic, ischemic or exercise induced stress. The results indicate that 13 genes (in pink) in other mouse models were either up-regulated or down-regulated in the same direction as that in the mild-SRF Tg mouse (labeled as “A” for agreement in the direction of change with the SRF Tg mouse). There were 20 genes (in purple) that showed a mixed pattern of being either “up- or down-regulated” (labeled as “A” for agreement or “D” for disagreement in the direction of change with the SRF Tg mouse) in the various models compared with the SRF Tg mouse. The other 15 genes (in light blue) were allaltered in the opposite direction to that which was observed in the mild-SRF Tg mouse (“D” for disagreement).
| Gene identifier | 6Mo SRF Tg vs. nTg | GDS488 7 day MI | GDS794 21 day TAC | GDS446 dnPI3K | GDS488 1 hr MI | GDS446 caPI3K | GDS144 5 wk exercise | GDS591 Doxy. (-), (tTA)+ | GDS591 Doxy. (+), (tTA)+ | GDS488 4 hr MI | GDS488 24 hr MI | GDS496 3 wk TNFa+ | GDS488 8 wk MI | GDS794 2 day TAC | GDS2258 female TAC | GDS1001 ATP1a1+/– | GDS2258 male TAC | GDS1302 3 ug/kg TCDD | GDS144 10 min exercise | GDS794 10 day TAC | GDS1247 Dysf –/– | GDS648 IGF1R+, caPI3K | GDS1302 6 ug/kg TCDD | GDS1302 1.5 ug/kg TCDD | GDS144 4 wk exercise | GDS2335 diabetic Endu | GD S2335 healthy Endu | GDS648 IGF1R+, dnPI3K | Percent agreement |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cwf19l1* | ↓ | A ↓ | A ↓ | 100 | |||||||||||||||||||||||||
| AA930519 | ↓ | A ↓ | 100 | ||||||||||||||||||||||||||
| Mup1* | ↓ | A ↓ | 100 | ||||||||||||||||||||||||||
| Myh4* | ↓ | A ↓ | A ↓ | A ↓ | A ↓ | A ↓ | 100 | ||||||||||||||||||||||
| Nppa | ↑ | A ↑ | A ↑ | A ↑ | A ↑ | 100 | |||||||||||||||||||||||
| Jag2* | ↓ | A ↓ | 100 | ||||||||||||||||||||||||||
| Fhl1 | ↑ | A ↑ | A ↑ | A ↑ | A ↑ | A ↑ | A ↑ | 100 | |||||||||||||||||||||
| Il15 | ↓ | A ↓ | A ↓ | A ↓ | A ↓ | A ↓ | 100 | ||||||||||||||||||||||
| Serpine1* | ↑ | A ↑ | A ↑ | A ↑ | 100 | ||||||||||||||||||||||||
| Pcsk5* | ↓ | A ↓ | 100 | ||||||||||||||||||||||||||
| Hspa1b* | ↑ | A ↑ | A ↑ | 100 | |||||||||||||||||||||||||
| Cyp2b10* | ↑ | A ↑ | 100 | ||||||||||||||||||||||||||
| Es1* | ↓ | A ↓ | 100 | ||||||||||||||||||||||||||
| Ift81* | ↓ | A ↓ | A ↓ | A ↓ | A ↓ | A ↓ | A ↓ | D ↑ | D ↑ | 75 | |||||||||||||||||||
| Ofa* | ↓ | A ↓ | A ↓ | D ↑ | 67 | ||||||||||||||||||||||||
| Cdca5* | ↓ | A ↓ | D ↑ | 50 | |||||||||||||||||||||||||
| Lancl2* | ↓ | A ↓ | D ↑ | D ↑ | 33 | ||||||||||||||||||||||||
| LOC547428* | ↓ | A ↓ | D ↑ | D ↑ | 33 | ||||||||||||||||||||||||
| R3hcc1 | ↑ | D ↓ | A ↑ | D ↓ | D ↓ | 25 | |||||||||||||||||||||||
| Tpm2 | ↑ | A ↑ | A ↑ | A ↑ | A ↑ | D ↓ | 80 | ||||||||||||||||||||||
| Myh7* | ↑ | A ↑ | A ↑ | A ↑ | D ↓ | 75 | |||||||||||||||||||||||
| Myl1* | ↑ | A ↑ | A ↑ | D ↓ | 67 | ||||||||||||||||||||||||
| Rab8b* | ↑ | A ↑ | D ↓ | D ↓ | 33 | ||||||||||||||||||||||||
| Emp1* | ↑ | A ↑ | A ↑ | A ↑ | A ↑ | A ↑ | D ↓ | D ↓ | 71 | ||||||||||||||||||||
| Emr1* | ↑ | A ↑ | A ↑ | D ↓ | A ↑ | D ↓ | 60 | ||||||||||||||||||||||
| Irf8 | ↑ | A ↑ | A ↑ | D ↓ | D ↓ | A ↑ | 60 | ||||||||||||||||||||||
| Tbp* | ↑ | A ↑ | D ↓ | 50 | |||||||||||||||||||||||||
| Postn # | ↑ | A ↑ | A ↑ | A ↑ | A ↑ | A ↑ | D ↓ | A ↑ | D ↓ | 75 | |||||||||||||||||||
| Il1f5* | ↓ | A ↓ | D ↑ | 50 | |||||||||||||||||||||||||
| Aqp4* | ↓ | A ↓ | D ↑ | A ↓ | A ↓ | 75 | |||||||||||||||||||||||
| Slc20a1* | ↑ | A ↑ | A ↑ | D ↓ | 67 | ||||||||||||||||||||||||
| Hpx* | ↓ | A ↓ | D ↑ | 50 | |||||||||||||||||||||||||
| Ugt2b5* | ↓ | A ↓ | D ↑ | 50 | |||||||||||||||||||||||||
| C77681* | ↓ | D ↑ | D ↑ | 0 | |||||||||||||||||||||||||
| Mup1* | ↓ | D ↑ | 0 | ||||||||||||||||||||||||||
| Anxa10* | ↑ | D ↓ | 0 | ||||||||||||||||||||||||||
| Dsc2* | ↓ | D ↑ | D ↑ | 0 | |||||||||||||||||||||||||
| Epb4.2* | ↑ | D ↓ | 0 | ||||||||||||||||||||||||||
| Crk* | ↓ | D ↑ | D ↑ | 0 | |||||||||||||||||||||||||
| Il4* | ↑ | D ↓ | 0 | ||||||||||||||||||||||||||
| Aqr* | ↑ | D ↓ | 0 | ||||||||||||||||||||||||||
| Brca1* | ↓ | D ↑ | 0 | ||||||||||||||||||||||||||
| E2f3* | ↓ | D ↑ | D ↑ | 0 | |||||||||||||||||||||||||
| Slc4a8* | ↓ | D ↑ | 0 | ||||||||||||||||||||||||||
| Serpina1d* | ↓ | D ↑ | D ↑ | 0 | |||||||||||||||||||||||||
| Cyp3a16* | ↓ | D ↑ | 0 | ||||||||||||||||||||||||||
| Akr1c6* | ↓ | D ↑ | 0 | ||||||||||||||||||||||||||
| Elovl2* | ↓ | D ↑ | D ↑ | 0 | |||||||||||||||||||||||||
| 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 83 | 80 | 76 | 75 | 75 | 67 | 57 | 50 | 50 | 50 | 45 | 45 | 43 | 33 | 0 | 0 | 0 | 0 | 0 |
Note: Columns from left to right: The 1st column shows the gene symbol. The 2nd column (in yellow) shows the direction of alteration of genes in the mild over-expression SRF Tg hearts. Columns 3–29 are a list of mouse models in the GEO database used for comparison. Experimental systems (columns) and genes (rows) were sorted so that those with the highest percentage agreement with the SRF Tg mouse in differentially expressed genes were toward the upper-left corner of the table (Table 3). The last column of the right and the last row at the bottom provides the percentage agreement of differentially expressed genes in the various models with the SRF Tg model.