Figure 17.2.

Melanoma metastasis to the liver. (A) NF-κB-Fluc reporter model of metastasis. 2 × 10⁵ of mouse melanocytes (NF-κB-Fluc⁺, H-RAS^V12+, INK4A/ARF^−/−) were intravenously injected into each nude mouse. Sixty days after injection, the bioluminescent images were obtained (upper panel), mice were euthanized and (B) histological analysis (H&E staining) of organs were performed, indicating melanoma metastasis to the liver. (C) 2 × 10⁵ of GFP-tagged melanocytes (H-RAS^V12+, INK4A/ARF^−/−)were intravenously injected into nude mice. Thirty days after cell injection, frozen sections were examined under the fluorescent microscope (10× magnification). H, hepatocytes; M, melanoma cells; RBC, red blood cells.