Figure 17.3.

Luminescent imaging of cultured dish. Hs294TIκB-Gluc reporter cells or Hs294T NF-κB-Gluc reporter cells were treated with the IKK inhibitor, BMS-345541 (0 to 10 mM) for 36 h. The plate containing the cultured cells and medium was subjected to bioluminescent imaging immediately following addition of 100 mM of coelenterazine. Two independent experiments were performed and similar results were achieved. Data showan increase in the level of IκBα (upper panels) and reducedNF-κB transcriptional activity (lower panels) upon treatment with BMS-345541.