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. Author manuscript; available in PMC: 2012 Apr 5.
Published in final edited form as: Cold Spring Harb Symp Quant Biol. 2011 Apr 5;75:403–418. doi: 10.1101/sqb.2010.75.038

Figure 7.

Figure 7

Isolation of pre-nucleosomal histone complexes. (A) Affinity purifications of CENP-A, H3CATD and histone H3.1 were conducted from chromatin-free extracts to identify CENP-A specific preassembly complexes (Foltz et al. 2009). Prenucleosomal CENP-A is uniquely associated with HJURP and NPM1 compared with histone H3.1. The CATD domain of CENP-A mediates the recruitment of HJURP as the H3CATD chimeric protein, which is recruited to centromeres, interacts with HJURP. (B) Model of the distinct histone chaperone complexes utilized by the histone H3 variants.