Skip to main content
. Author manuscript; available in PMC: 2012 Apr 5.
Published in final edited form as: Cold Spring Harb Symp Quant Biol. 2011 Apr 5;75:403–418. doi: 10.1101/sqb.2010.75.038

Figure 8.

Figure 8

CENP-A assembles into octameric nucleosomes with conventional handedness of DNA wrapping (Sekulic et al. 2010). (A) Histone content of assembled H3- and CENP-A-containing nucleosomes. (B) Digestion of nucleosome arrays with micrococcal nuclease reveals that both H3-and CENP-A-containing nucleosomes protect ~150 bp of DNA. (C–F) Topological analysis of H3-(C and D) and CENP-A-containing (E and F) nucleosomes. Analysis by gel electrophoresis in the absence (C and E) or presence (D and F) of chloroquine reveals that both H3- and CENP-A-containing nucleosomes wrap DNA in a left-handed manner.