Figure 2.
Typical workflows for mass spectrometry–based biomarker discovery and validation. Abbreviations: FTMS, Fourier transform mass spectrometry; HPLC, high-performance liquid chromatography; ICAT, isotope-coded affinity tag; IMAC, immobilized metal affinity chromatography; ITRAQ, isotopic tag for relative and absolute quantification; LTQ, linear trap quadrupole; MS/MS, tandem mass spectrometry; RP, reverse-phase liquid chromatography; SCX, strong cation-exchange liquid chromatography; TOF, time of flight.
