Skip to main content
. 2011 Jul 20;6(7):e21679. doi: 10.1371/journal.pone.0021679

Figure 2. Mir-145 directly targets Sox9.

Figure 2

(A) A schematic shows that the 5′ end of miR-145 contains a sequence complementary to the specific miRNA binding site within the 3′-UTR of Sox9 mRNA. (B) Pre-miR-145 or its negative control was co-transfected with the specific pMIR-REPORT construct containing a consensus miR-145-binding site (pMIR-PT) into HEK293 cells. Pre-miR-145 or its negative control was co-transfected with pMIR-MRE into HEK293 cells. pMIR-PT acts as a positive control. (C) HEK293 cells were co-transfected with pMIR-MRE together with varying amounts of pre-miR-145, as indicated. Statistical comparisons were made between multiple groups by ANOVA. (D) Anti-miR-145 or its negative control was co-transfected with either pre-miR-145 together with pMIR-MRE or pre-miR-145 together with pMIR-MUT into HEK293 cells. pMIR-PT acts as a positive control. All cells (B, C, D) were harvested at 48 h after transfection, and then luciferase activities were measured and normalized to the phRL-TK activities. Three independent transfection experiments were done and data was represented as mean±sd. *, p<0.05, **, p<0.01, when compared with control.