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. 2011 Jul 20;6(7):e22164. doi: 10.1371/journal.pone.0022164

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Deghorain et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The dif-lacI dif cassette is shown with the dif sites as black and white squares. It was inserted at chosen loci of a Δ(xerC)Δ(dif)Δ(lacI) strain. Transformation with the XerC-producing plasmid pFC241 allows recombination, giving rise to blue colonies on indicator medium (right). (B) Map of the chromosome with the insertion loci used for insertion of the dif-lacI dif or parS-Kn cassettes. The replication origin (closed circle) and replication terminators (black flags) are indicated. Coordinates are in Kb.