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. 2011 Jul 20;6(7):e22554. doi: 10.1371/journal.pone.0022554

Figure 5. Var26 prevents polyQ-induced cell death.

Figure 5

A. SH-SY5Y cells were cotransfected with pcDNA-HttEx1(25Q) or (97Q)-GFP together with an empty vector (Vec) or, pcDNA3-WT 3CP (WT), pcDNA3-Var26 (Var26), or pcDNA3-C172A (C172A). After 48 hrs of incubation, the cells were stained with trypan blue and viewed under a microscope. Arrows indicate cells stained with trypan blue. The percentage of dead cells was plotted (n = 3). Error bars represent the SD. Significance was determined by student's t test. *p<0.05, **p<0.01. B. In a different experiment, the same group of cells was examined for, pyknotic nuclei by Hoechst staining. The number of pyknotic nuclei was measured and is plotted (n = 3). Error bars represent the SD. Significance was determined by student's t test. *p<0.05, **p<0.01. C. Cells harboring the plasmids described in panel A were lysed, separated by SDS-PAGE, and blotted and probed with a caspase-3 antibody. Tubulin was used as a loading control.