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. 2011 Jul 20;6(7):e22502. doi: 10.1371/journal.pone.0022502

Figure 2. The impact of SCF withdrawal on HIF-1α accumulation and the GSH-dependent antioxidative system in LAD2 human mast cells.

Figure 2

LAD2 cells (which were continuously cultured in the presence of 100 ng/ml SCF) were incubated in fresh media containing 100 ng/ml SCF for 24 h, while another fraction of these cells was subjected to SCF-free media for 24 h. (A) HIF-1α accumulation, GSH and GSSG levels, GPx/GR activities and the quantity of the TBRS were then measured as outlined in Materials and methods. HIF-1 DNA-binding activity was also analysed. (B) Quantitative data are mean values ± S.D. of at least three individual experiments. *P<0.01 vs control. Western blot data are shown from one representative experiment out of three that gave similar results.