Figure 4. GSH depletion leads to a strong decrease in SCF-dependent HIF-1α PHD activity in LAD2 cells.

LAD2 cells were incubated for 24 h in fresh media ±100 ng/ml SCF and ±500 µM BSO. (A) HIF-1α accumulation and PHD activity were assessed as well as (B) Annexin V and DAPI staining as outlined in Materials and methods. Western blot and imaging data are from one representative experiment out of three. Quantitative data are mean values ± S.D. of at least three individual experiments. *P<0.01 vs control.