Serum samples (A-D) and Spleen cell culture (E-H) were prepared as described in the Materials and Methods from C57BL/6 mice following i.p.infection with either a high dose of IOE (lethal) or E. muris (non lethal). IL-18 (A, E), IL-12 (B, F), IFN-γ (C, G), and IL-10 (D, H) were measured in sera or spleen culture supernatant at different time points after infection by ELISA. The data shown represent the mean ± SEM of three mice per group. The data are representative of two independent experiments with similar results. Significant differences between lethally and non-lethally infected mice were assessed. *P <0.05, **P< 0.01. , ***P< 0.001