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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Immunol. 2011 Jun 29;187(3):1333–1346. doi: 10.4049/jimmunol.1100092

FIGURE 12. The lack of IL-18Rα signals causes downregulation of T cell costimulatory functions of dendritic cells during severe ehrlichial infection.

FIGURE 12

A, Splenocytes were harvested from IOE-infected IL-18Rα-/- and WT mice on day 3 p.i., and the expression levels of CD40 and MHCII costimulatory molecules on CD11c+ dendritic cells were analyzed by flow cytometry. B, Bone marrow derived dendritic cells (BMDCs) were isolated from WT and IL-18Rα-/- mice and were propagated in vitro. Cells were either left unstimulated or infected with IOE at MOI of 5: 1 for 24 hours. Histogram show different maturation markers; MHC-I, MHC-II, CD80, CD86, and CD40 on CD11c+ BMDCs from naive (filled line), WT (solid line) and IL-18Rα-/- (dotted line). C, TNF-α and IL-6 production in culture supernatant of IOE-infected BMDCs from WT and IL-18Rα-/-. Uninfected BMDCs from WT mice was used as negative controls. In vivo experiments showing results from four mice/ group, and similar results were obtained in three independent experiments. In vitro data are representative from one of three different experiments with similar results.