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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Immunol. 2011 Jun 27;187(3):1222–1234. doi: 10.4049/jimmunol.1100469

Figure 6. Cytosolic DNA activation of DCs partly depends on the IFI16 sensor.

Figure 6

A, IFI16 gene expression upon DC maturation with different activation stimuli. B, Effectiveness of IFI16-specific knock-down. C, IFN-β gene expression by siNA-treated DCs measured after 4h of p(dA:dT) activation by Q-PCR. D, CD80, CD83 and CD86 surface expression of the siRNA-treated, DNA-activated DCs. Dashed line: isotype control, grey thin line: IDC, black thin line: DNA-DC treated with control siRNA, thick line: DNA-DC treated with IFI16-specific siRNA. E, IL-6 and TNF production by siRNA-treated DNA-DCs. F, Total allogeneic T cells were co-cultured with IDCs or siRNA-treated, poly(dA:dT)-activated DCs and IL-2 and IFN-γ cytokine production by T cells was measured by ELISA.

n=3 independent experiments.