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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Immunol. 2011 Jul 1;187(3):1289–1297. doi: 10.4049/jimmunol.1003399

Figure 1. TNF induced T cell morphological change is serine phosphorylation dependent.

Figure 1

(A) Peripheral T cells and peripheral B cells obtained from healthy blood donors were cultured in the chambers coated with ICAM-1 in the presence or absence of TNF (200 ng/ml) with or without serine kinase inhibitor H7 pretreatment for 1hr and immediately subjected to time-lapse fluorescence imaging at 37°C. Cell morphological changes were recorded at time 0 or 30 min after. (B) Peripheral T cells and peripheral B cells obtained from healthy blood donors were cultured in the chambers coated with ICAM-1 in the presence or absence of TNF (50 ng/ml) with or without different dose of serine kinase inhibitors H7 or staurosporine pretreatment for 1 hr and immediately subjected to time-lapse fluorescence imaging at 37°C. Cell numbers with morphological changes were counted after 30 min. Data presented are mean ± SD. of triplicate determinations.