Axotomy increased the number of Nav1.1-positive neurons in the L5 dorsal root ganglia (DRG). Representative images of naive DRG (A), ipsilateral (Ipsi) L5 DRG (B), and contralateral (Contra) L5 DRG (C) on day 7 after sciatic nerve axotomy. Insets a–c are high magnification images of the Nav1.1-positive cells indicated by the arrows in A–C, respectively. Scale bars: 200 µm in A–C and 20 µm in a–c. D: Statistical summary showing the percentage of Nav1.1-positive neurons in naïve (D0) DRG, ipsilateral L5 DRG (B), and contralateral L5 DRG (C) on day 7 (D7) after sciatic nerve axotomy. * P < 0.05 vs the corresponding naive group. # P < 0.05 vs the corresponding contralateral side.