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Splenocytes of unimmunized (A, C) or ML1419c p113-121-immunized (B, D) HLA- A2 tg mice were stained with PE-conjugated anti-CD8 and APC-conjugated HLA-A*0201/ml1419c p113-121 tetramer (HLA-A2/p113 TM) directly ex vivo (A, B) or after 1 week in vitro restimulation with ML1419c p113-121 (C, D).
