Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Reprod Dev. 2011 Jun 27;78(7):519–528. doi: 10.1002/mrd.21342

Figure 3.

Figure 3

Sorbitol induces an early peak of Eomes at 60 to 120 minutes that subsides by 4hr and returned to below baseline at 24hr. TSCs were cultured in 200mM Sorbitol for 0–24hr, lysed, and fractionated by SDS-PAGE, and immunoblotted for Eomes or stained by amido black as a loading control. In the histogram, error flags show the standard deviation for three experiments. Overall ANOVA shows significant Eomes induction for all Sorbitol durations between 60 and 120min when compared with unstressed TSCs at 0min, 30mins, 4hr and 24hr. Post hoc Dunnett t test shows Eomes is induced significantly at 60min–120min (a) (p<0.001), but that Eomes is not significantly different in the Sorbitol-treated group at 4hr. Post hoc Dunnett t test shows no difference for Eomes when TSCs are stimulated by sorbitol for 24hr (b) compared with unstressed TSCs at 0min.