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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Immunol. 2011 Jun 20;187(3):1207–1211. doi: 10.4049/jimmunol.1100355

Figure 1.

Figure 1

DCs express TSLP in response to TLR stimulation and IL-4 can augment this induction. (A and B) Bone marrow derived DCs were stimulated for 4 hr with various TLR ligands, and TSLP (A) and TNFα (B) mRNA levels relative to RPL7 were determined. (C–E) Bone marrow derived DCs were stimulated with zymosan (C), LPS (D), or CpG (E) for varying time points and TSLP mRNA expression was assessed. (F) Purified splenic DCs were activated with LPS and CpG for varying time points and TSLP mRNA expression was assessed. The data are representative of 3 independent experiments. (G and H) IL-4 augments TSLP mRNA and protein production in bone marrow-derived DCs. On day 8, WT or Stat6 KO DCs were activated with zymosan, LPS, or CpG, without or with IL-4 for 4 or 16 hr and mRNA and supernatant harvested for TSLP RT-PCR (G) and ELISA (H), respectively. The data are representative of 3 independent experiments. *, p < 0.05 and **, p < 0.01 as compared to untreated samples (A–F) or as indicated (G,H).