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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Immunol. 2011 Jun 20;187(3):1207–1211. doi: 10.4049/jimmunol.1100355

Figure 4.

Figure 4

Lung DCs produce TSLP upon HDM challenge. BALB/c mice were injected with HDM as described in the Materials and Methods. (A–D) BAL fluid cellularity was determined (A), and the percentages of neutrophils (B), T cells (C), and DCs (D) were determined by flow cytometric staining with Ly-6G/CD11b (B), TCRβ (C) CD11c/CD11b (D). The data are combined from 5 independent experiments with 3–5 mice in each group yielding a total of 18 mice. (E and F), TARC (E) and TSLP (F) protein levels were measured by ELISA in lung homogenate. Data are from 1 of 3 similar experiments, each of which had 3–5 mice in each group. (G–I) TSLP and TARC mRNA levels are induced by HDM in lung DCs. CD324+CD11c− (epithelial cell) and CD324+CD11c+ (dendritic cell) populations of total pooled cells from 5 mice were isolated by cell sorting from PBS or HDM-treated mice, mRNA was isolated and RT-PCR performed for TSLP, TARC and Pulmonary surfactant-associated protein B (SFTPB). Data are expressed as the ratio of TARC, TSLP, or SFTPB mRNA to that of the RPL7 housekeeping gene and are from 3 independent experiments. *, p < 0.05 and **, p < 0.01 for HDM vs. PBS.