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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Immunol. 2011 Jun 22;187(3):1113–1119. doi: 10.4049/jimmunol.1100056

Figure 7. The interaction of PDL1 on APCs with B7.1 on T cells plays a dominant role in bidirectional interactions between these two molecules.

Figure 7

Fourteen days after B6 WT skin was transplanted into Balb/c WT or PDL1KO recipients, spleens and lymph nodes from recipients were harvested and CD4+ T cells were isolated. CD4+ T cells were then cultured with irradiated CD11c+ cells from Flt-3L-injected B6 WT or PDL1KO mice. Cell-free supernatants of individual wells were removed after 48 h of incubation with 2H11 or control IgG and analyzed by a Luminex for IFN-γ production. 2H11 mildly increased IFN-γ-production by CD4+ T cells when PDL1 was present on both APCs and T cells (A), however no effect was seen when PDL1 was absent on both cells (B). In addition, 2H11 did not enhance IFN-γ production when PDL1 was absent on CD11c+ cells alone (D). 2H11 increased IFN-γ production when PDL1 was present on the APC side but absent in T cells (C). Bar graphs show means ±SEM. All results are representative of at least three different sets of experiments with 3 mice.