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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Nat Immunol. 2011 Jun 19;12(8):752–760. doi: 10.1038/ni.2052

Figure 3. Pharmacological inhibition of InsP6K activity augments PtdIns(3,4,5)P3 signaling and NADPH oxidase-mediated superoxide production in human primary neutrophils.

Figure 3

(a) Immunoblot analysis of total and phosphorylated Akt in TNP (10μM)-treated and untreated human neutrophils stimulated with 1μM fMLP. The results shown are representative of three experiments. Relative amounts of phosphorylated Akt were quantified with NIH Image software. (b) fMLP-elicited extracellular ROS production in DMSO and TNP-treated human neutrophils. (c) fMLP-elicited intracellular ROS production in human neutrophils. (d) C5a -elicited extracellular ROS production in human neutrophils. (e) C5a-elicited intracellular ROS production in human neutrophils. Data are the means (±SD) of three independent experiments. *p < 0.01 (Student's t test).