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Parallel preparation of seesaw gates as hairpin precursors. Using DNA microarray synthesis technologies, each gate, threshold and fuel is made as a single strand. After cleavage from the surface, the mixture is annealed to form hairpins. Restriction enzymes then cleave the hairpins to form gate and threshold complexes. Relative concentrations (red numbers) are set based on the number of DNA chip spots dedicated to a given strand; in this example, one spot produces 0.25x.
