Skip to main content
. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Clin Cancer Res. 2010 Dec 21;17(6):1362–1372. doi: 10.1158/1078-0432.CCR-10-2213

Figure 3. Chase expression from OV-Chase does not interfere with viral cytotoxicity.

Figure 3

Glioma cells (U343, LN229 and U87ΔEGFR) were inoculated with rHsvQ or two different isolates of OV-Chase (OV-Chase 1 and OV-Chase 2) at MOI = 0, 0.05, 0.1, 0.5 or 1. Two independent isolates of OV-Chase were purified and used in this assay to test if changes in virus infection or replication were specific to the inserted Chase ABC expression cassette. Cell viability was assessed during five days post-infection, using a standard crystal violet assay. Columns (% of surviving cells at day 5) indicate mean values for each treatment (± 95% CIs) (n=4/treatment for each cell line). Results indicate that in vitro cytotoxicity of both the independent isolates of OV-Chase is comparable to control rHsvQ.