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. 2011 May 19;20(16):3138–3150. doi: 10.1093/hmg/ddr216

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Figure 5. — Chd7^Gt/+ embryos have reduced cellular proliferation in the olfactory placode. (A–F) Immunofluorescence of the wild-type and Chd7^Gt/+ at E10.5 using antibodies against CHD7 (red) and phospho-histone H3 (H3) (green). (G–I) Immunofluorescence of Chd7^Gt/Gt embryos at E10.5 using antibodies against CHD7 (red) and phospho-histone H3 (H3) (green) counterstained with DAPI (blue). (J) Cell counts showed decreased cellular proliferation in the olfactory placode in Chd7^Gt/+ and Chd7^Gt/Gt embryos compared with wild-type littermates. (K) Cell counts showed a decrease in TUNEL-positive cells at E10.5, E11.5 and E12.5 in Chd7^Gt/+ embryos compared with the wild-type. Sections are in the transverse plane. **P < 0.01, ***P < 0.001 by ANOVA (proliferation) and unpaired Student's t-test (TUNEL).