Figure 6.

Reduced CHD7 dosage alters gene expression in olfactory placode, adult hypothalamus and adult pituitary. TaqMan gene expression assays were done on microdissected tissues. (A) Expression of Otx2, Bmp4 and Fgfr1 is decreased in E10.5 Chd7^Gt/+ and Chd7^Gt/Gt olfactory placode compared with wild-type littermates, whereas Fgf8 expression is unchanged. (B) Expression of Otx2 and GnRH1 is decreased in Chd7^Gt/+ hypothalamus compared with wild-type littermates, whereas Kiss1 expression is unchanged. Expression of GnRHR is decreased in Chd7^Gt/+ pituitary compared with wild-type littermates. *P< 0.05 by DataAssist Software.