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. 2011 May 15;20(16):3161–3175. doi: 10.1093/hmg/ddr218

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© The Author 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Gene therapy improves cone survival in Cngb3^−/− mice. Injections were performed at P15 and eyes were collected at 60 days post-injection for PNA lectin cytochemistry and analyses of cone-specific protein expression. (A) PNA labeling and cone-opsin staining in the treated Cngb3^−/− eyes, compared with the untreated control eyes. Retinal sections were analyzed for: (a) PNA lectin staining, (b) M-opsin and (c) S-opsin immunostaining. Shown are representative images of the analysis from 4 to 6 mice/group and correlative quantitative results. OS, outer segment; ONL, outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bar: 50 μm. Quantitative results were obtained from 14 to 18 retinal sections prepared from four mice in each group. Unpaired Student's t-test was used for determination of the significance (P < 0.01). (B) Expression of M-opsin, S-opsin, CAR and GNAT2 in the injected-eyes analyzed by western blot analysis. Retinal membrane preparations were resolved by 10% SDS–PAGE, followed by immunoblotting using antibodies against the respective proteins. Shown are representative images of the analysis from 6 to 8 mice/group.