Skip to main content
. Author manuscript; available in PMC: 2011 Jul 21.
Published in final edited form as: J Invest Dermatol. 2009 Nov 5;130(3):755–762. doi: 10.1038/jid.2009.343

Figure 1. Langerhans cells in situ are specifically targeted by mAb in murine skin explant cultures.

Figure 1

(a): Whole skin explants from BALB/c mice were incubated for 30′ to 48h in culture medium containing NLDC145 (anti-mouse DEC-205) hybridoma supernatant. Epidermis was then separated from dermis, fixed, and targeting mAb was revealed with anti-rat IgG secondary Ab. Arrowheads indicate dermal cells targeted in situ. Arrows point at DC-filled dermal lymphatic vessels. (b): Whole skin explants from BALB/c mice were incubated for 18 h in culture medium containing NLDC145 or LODNP16 (isotype control) hybridoma supernatants, then epidermis was separated, fixed, and stained with anti-rat IgG Ab. (c): Whole skin explants from BALB/c or C57BL/6 mice were incubated for 4h with 5μg/mL of the indicated purified mAb, then epidermis was separated, fixed, and stained with anti-rat IgG Ab. Scale bars = 100μm. Results are representative of three independent experiments.